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Phosphofructokinase 1

Enzyme that regulates the speed of glycolysis.

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Phosphofructokinase 1

Enzyme that regulates the speed of glycolysis.

It is a transferase enzyme that catalyzes fructose 6-phosphate + ATP → fructose 1,6-bisphosphate + ADP, with Mg²⁺ as a cofactor. This essentially irreversible reaction is a commitment step in glycolysis.

Phosphofructokinase 1 is a major control point for glycolytic flux and responds to cellular energy needs. Its allosteric regulation coordinates glucose breakdown with the availability of ATP and other metabolites.

The video explains that high ATP and citrate inhibit it, whereas AMP, ADP, and fructose 2,6-bisphosphate activate it. These signals indicate whether the cell already has enough energy and adjust the rate of the pathway.

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