Entity
DNA polymerase III
Enzyme that synthesizes most of the new DNA during bacterial replication.
It is the main enzyme that synthesizes new DNA during bacterial replication, adding nucleotides complementary to the template strand in the 5′ to 3′ direction.
It is part of a multiprotein complex, the replisome, and works together with a sliding-clamp processivity subunit that keeps it attached to the DNA. It always requires a primer to start synthesis, since it cannot begin a strand de novo.
The video explains that it needs the primer placed by primase to start adding nucleotides to the template strand, synthesizing the leading strand continuously and the lagging strand in Okazaki fragments, since it needs a new primer each time.
Relations
- DNA polymerase III → extends the strand in → DNA replication
Evidence
Sources · original ES transcript
- The Rule Behind All Life | The Central Dogma6:01
añadir nucleótidos complementarios a la cadena molde
- The Rule Behind All Life | The Central Dogma6:01
- DNA polymerase III → forms → Okazaki fragments
Evidence
Sources · original ES transcript
- The Rule Behind All Life | The Central Dogma6:21
formando pequeños fragmentos llamados fragmentos de Okazaki.
- The Rule Behind All Life | The Central Dogma6:21
Sources · original ES transcript
- The Rule Behind All Life | The Central Dogma6:01
La DNA polimerasa III necesita del primer para poder comenzar a añadir nucleótidos complementarios a la cadena molde, siempre haciéndolo en dirección 5′ a 3′.
- The Rule Behind All Life | The Central Dogma6:14
En la cadena líder, la síntesis es continua, ya que la DNA polimerasa solo requiere de un primer, pero en la cadena rezagada necesita más de uno, formando pequeños fragmentos llamados fragmentos de Okazaki.